Measurement of environmental contamination in water using bioluminescence systems is known as bioluminescence inhibition assay (BIA). Bioluminescence in these bacteria is regulated by quorum sensing, and BIA is a rapid toxicity assay based on the reduction of bacterial light emission after exposure to toxic compounds. This study was conducted to introduce a native strain with the aim of using bioluminescence for rapid and simple water contamination detection. For this purpose, Vibrio fischeri (standard strain) and a native Iranian species (Vibrio sp. Persian1) were cultured in different media (ASW, PGSW and CSW) under optimized conditions and used by BIA in order to monitor water contamination. The results of this study showed that a culture medium of artificial seawater (ASW) was suitable for growth and emission of light by these bacteria after 4 hours. The light emission of each bacterial strain was measured at 0 and 5 minutes, with three independent replicates. For this, 𝑍𝑛𝑆𝑂4 was used as a model toxicant contaminant that may be present in water, with concentrations ranging from 0.25 to 1 M. This bioluminescence-based assay is currently used in various countries for monitoring and assessment of water pollutants. As a result, Vibrio sp. Persian1, which is native to our climate, can be used for rapid, simple, and cost-effective detection of environmental pollution in water under optimal conditions, which reduces energy consumption. Studies have indicated that in the presence of 1M zinc sulfate, Vibrio fischeri and Vibrio sp. Persian1 showed a 41% and 34% reduction, respectively.